Crystallin promoter

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August undefined, 2024

WebBy use of a gene fusion of the alpha A-crystallin promoter to the viral immortalizing oncogene, polyoma large T antigen (PyLT), we created transgenic mice that express PyLT specifically in ocular lens. Expression of large T antigen during embryonic development led to a perturbation in lens development, specifically, an interference with the ...

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WebMar 1, 1989 · Recently Landel et al. (1988) have combined the α -crystallin promoter with the ricin cytotoxic gene to produce a single line of transgenic mice with a somewhat more severe phenotype consisting of a still smaller lens and other interesting eye malformations. WebA conserved and essential DNA motif in the promoters of the γ-crystallin genes is the binding site of the SOX proteins that activate the promoters (Kamachi et al., 1995). … grace bubble bath

Regulation of mouse lens fiber cell development and differentiation by ...

WebThe beta-B1 crystallin promoter produced GFP expression in the lenses of 37% of injected embryo’s after 48 hours. Fifty percent of MO injected … WebRetrospectively from the results of ( Maf) knock-out mice. it was also shown that MAF binds the promoter sequences of some, B-crystallin promoters ( Ring et al., 2000 ). Three … WebDec 1, 2012 · The gene promoter contains response elements for some key transcription factors essential for normal lens development. Prominent among these are factors such as Pax6, c-Maf, Sox2, Prox1, and Six3 [93,94]. In many crystallin gene promoters, elements for lens and non-lens expression are combined in the same 5 ′ region. chili\\u0027s reviews

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Transcriptional Regulation of the Mouse αA-Crystallin Gene: …

WebDec 3, 2013 · CpG island methylation in the αA-crystallin promoter was similar in age-related cataract and high-myopic cataract classified as Lens Opacity Classification System III Grade Nuclear Color 2–3. Pyrosequencing, a real-time DNA sequencing method, was used to determine single-base variations caused by CpG methylation of the CRYAA … WebThe two paralogous zebrafish αB-crystallin promoters produced subtly different expression profiles, with the aBa promoter driving expression equally in notochord and skeletal muscle while the αBb promoter resulted primarily in skeletal muscle expression. grace buchananWebFGF2 strongly activates expression of the αA-crystallin gene promoter coupled with the DCR1 enhancer compared to the promoter alone. 234 Interestingly, FGF signaling also regulates expression of a large set of miRNAs that modulate gene expression during FGF2-induced lens fiber cell differentiation. 157 At least three miRNAs, including miR-143 ... chili\\u0027s rewards log in

"WebThe inclusion of a Pax6 consensus binding site within the -282/+43 alphaA-crystallin promoter enhances the ability of this promoter to drive transgene expression in the lens … " - Crystallin promoter

Crystallin promoter

Evolutionary Origins of Pax6 Control of Crystallin Genes

WebProx1 activates several of the different crystallin promoters, and acts synergistically with another lens transcription factor, Maf, to activate transcription of ß1 and ß2 crystallins (Lengler ... WebSox-1 transactivated the γF-crystallin promoter in a dose-dependent fashion, similar to c-Maf (Fig. 2 C). Sox-1 also stimulated the βB2 promoter, but less strongly than c-Maf (Fig. 2 B). Prox-1 (80 ng) stimulated the βB2- and γF-crystallin promoters only about 2-fold (Fig. 2, B and C). To determine whether these transcription factors can ...

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WebSep 1, 2002 · These data suggest that the chicken β B1-crystallin promoter is the promoter of choice to direct the high level ectopic expression of RNAs to lens fiber cells … WebThe J3-crystallin promoter (Kozmik etal. 2003) contains a pair of PaxB/Pax6-binding sites though its complete promoter sequence remains to be reported. Finally, two squid (Ommastrephes sloani) crystallin promoters have been characterized using vertebrate lens nuclear extracts (Tomarev etal. 1994). The SL11 and SL20 crystallin promoters …

WebJul 5, 2002 · Crystallin promoter reporters. The constructs with the αA-, βB2-, and γF-crystallin promoters linked to a luciferase reporter are depicted. Potential c-Maf recognition sites are located at −110 to −98 in the αA and βB2 promoters, and at −47 to −35 in the γF promoter ( 30, 31, 32 ). WebCrestline serves sophisticated institutions, family offices and high-net-worth individuals in North America, Europe and Asia who seek innovative alternative investment solutions …

WebJul 15, 1992 · Progeny from two of these lines were mated with transgenic mice expressing the Cre recombinase under control of either the murine alpha A-crystallin promoter or … WebMar 21, 2024 · Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell …

WebPromoter methylation of RASEF was associated with increased risk of death due to metastasis, ... Epigenetic regulation of αA-crystallin in high myopia-induced dark nuclear cataract. PLoS One 2013; 8: e81900. doi: 10.1371/journal.pone.0081900. 8. Mao W, Rubin JS, Anoruo N, Wordinger RJ, Clark AF.

WebApr 8, 2024 · Further explorations unveiled the increased MAF in high myopia and its participation in a direct activation of crystallin promoters and an indirect activation via … chili\u0027s reviews by locationWebA DCR1/αA-crystallin promoter (including DCR2) coupled with EGFP virtually recapitulated the expression pattern of αA-crystallin in lens epithelium and fibers. In contrast, the DCR3/αA/EGFP reporter was expressed only in 'late' lens fibers. Chromatin immunoprecipitations showed binding of Pax6 to DCR1 and the αA-crystallin promoter … chili\u0027s restaurant tarpon springs flWebNov 3, 2003 · In order to identify PaxB response elements in the J3-crystallin promoter, we generated a series of J3 promoter deletions in the luciferase reporter construct. The shortest promoter fragment, consisting of only 200 bp upstream of the transcriptional start site, was activated by PaxB and not by PaxB(I84R) (data not shown; but cf. Figure 5C ) . grace buchele mineta babyWebEarlier experiments showed that the betaB1-crystallin promoter sequence from -120 to -48 contains a cis element (PL2 at -90 to -76) that stimulates the activity of a heterologous promoter in lens cells but not in fibroblasts. grace bubble writingWebThis study concluded that there existed a TGF-β responsive element in the promoter just 285 base pairs upstream of the transcriptional start site ... porcine TGF-β1 under the lens-specific αA-crystallin promoter and then another similar line was created but with the gene driven by the βb-crystallin promoter, corresponding to another lens ... chili\u0027s restaurant woodbury mnWebFeb 21, 2024 · The sequence between SacI and AfeI was replaced with a sequence containing CG-depleted chicken lens δ1-crystallin gene (Sasaki) promoter 51, a … grace bucheleWebThis present study is the first to show that mammalian α-crystallin promoter function can be analyzed in zebrafish embryos by observing green fluorescent protein (GFP) … grace bucket