Web11 apr 2024 · After centrifugation, the supernatant was gatherd and loaded onto the HisTrap HP column (Cytiva) and eluted with Buffer B (50 mM Tris-HCl (PH = 8.0), 600 mM imidazole, 1.5 M NaCl). Web27 mag 2024 · 故障排除 16 10. 定购信息 20 附录A 22 3 1 说明 介质的性质 HisTRap FF crude 1-ml 和 5-ml 柱是用亲和介质 Ni Sepharose 6 Fast Flow 的预装柱，由具有 固定鳌合基团的高度交联的球状琼脂糖组成。. 该介质已预荷有Ni2＋离子电荷。. 几种氨基酸，例如，组氨酸能与许多金属离子 ...

HisTrap FF, 1mL and 5mL

Webml/min or 5 ml/min for 1 ml and 5 ml column respectively. 5 Apply the sample, using a syringe fitted to the luer connector or by pumping it onto the column. 6 Wash with 5 to 10 column volumes of binding buffer or until no material appears in the effluent. 7 Elute with 2 to 5 column volumes of elution buffer. Other volumes Web17-5255-02 HisTrap FF 5 ml 100 × 5 ml * * Special pack size delivered on specific order. Connectorkit Connectors supplied Usage No. supplied switchere supported countries

HisTrap™ Excel Cytiva 17-3712-06, pack of 5 × 5 mL - Sigma-Aldrich

WebHiTrap 1 mL: 4 mL/min HiTrap 5 mL: 20 mL/min Recommended operating flow rate HiTrap 1 mL: 1 mL/min HiTrap 5 mL: 5 mL/min Chemical stability Stable to commonly used … Web17-0851-01 1.0–2.5 ml 1 1.75–2.5 ml 2 3.5 ml 1 Up to 2.5 ml 2 Prepacked with Sephadex G-25 Medium. Runs by gravity flow or centrifugation For desalting, buffer exchange, and cleanup of proteins and other large biomolecules (M r > 5000). PD MiniTrap™ G-25 28-9180-07 0.1– 0.5 ml 1 0.2–0.5 ml 2 1.0 ml 1 Up to 0.5 ml 2 PD MidTrai p™ G-25 WebTable 1. HisTrap FF crude characteristics Matrix Highly cross-linked spherical agarose, 6% Average bead size 90 µm Metal ion capacity ~15 µmol Ni2+/ml medium Dynamic binding capacity* Approx. 40 mg (His) 6-tagged protein/ml medium Column volumes 1 ml or 5 ml Column dimensions i.d. × H: 0.7 × 2.5 cm (1 ml) 1.6 × 2.5 cm (5 ml) switchere coupon code