Orbitally shaken
WebOrbitally shaken bioreactors (OSBs) utilize the same hydrodynamic principles that define the success of smaller-scale cultur … Increasing the cultivation volume from small to large … WebJun 15, 2012 · The volumetric power input of the 200 L and 2000 L orbitally shaken bioreactors (Kühner AG, Birsfelden, Switzerland) was measured applying a slightly modified temperature method. The temperature method is based on the fact that the volume specific heat energy per time produced by the friction of the liquid on the walls of the bioreactor is ...
Orbitally shaken
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WebNov 16, 2024 · Disposable orbitally shaken bioreactors have been widely used for mammalian cell culture in suspension. Three kinds of baffle structures: vertical baffle, inclined baffle and horizontal baffle were designed in this work. The flow fields of the shaking bioreactor with different baffle structures were simulated, and the turbulence, … WebAug 12, 2024 · This study investigated the efficacy of a novel correlation of power input, energy dissipation rate and mixing time as a potential route to identify the orbitally …
WebJan 15, 2014 · At laboratory scale, cells are usually grown in low shear devices, with orbitally shaken bioreactors (OSRs) being largely employed in the early stages of bioprocess … Web: Orbitally shaken single-use reactors are promising reactors for upstream processing, because they fulfill three general requirements for single-use equipment. First, the design …
WebNov 21, 2024 · Here, we report the implementation of a scalable process for the production of AAV vectors using suspension HEKExpress cells in orbitally shaken bioreactors (OSRs) and polyethylenimine (PEI)-mediated transient transfection. WebAug 19, 2024 · The orbitally shaken bioreactors (OSRs) were shaken on a platform connected to a shaker motor. The distance between the attachment point of the platform and the center axis of the shaker motor represents half the shaking diameter (= displacement radius). The OSRs contained deionized water maintained at 37 °C.
WebShaken bioreactors were comprehensively utilized in the early stages of bioprocess development and microscale plates (24-, 48-, 96- or 384-orifice discs) facilitated a significant number of experiments. The shaker (25 mL–6 L) was the most widely used orbitally shaken bioreactor (OSB) type, providing a
WebThe SB10-X is an orbital shaken benchtop bioreactor system for the cultivation of human, mammalian and plant cells in a single-use bag. It has a working volume from 4 litres up to … how far is coralville from iowa cityWebExhibitor. Kuhner shaker, founded in 1949 in Basel, Switzerland, is a science-first shaker manufacturer renowned worldwide for our uncompromising shakers, incubator shakers, and orbitally shaken bioreactors complying with GMP regulations. From bench top shakers to large scale industrial shaking machines, we offer machines of the highest quality. how far is cordova from memphis tnWebJan 22, 2010 · An overview of the disposable bioreactors that are currently commercially available is provided, and the domination of wave-mixed, orbitally shaken, and, in particular, stirred disposable bioreactors in animal cell-derived productions at … how far is cordova from memphisOrbitally shaken bioreactors are widely used for small-scale screening and process optimization. Complex mechanical and electronic parts such as the shaker drive, the power train, and the control unit are integrated in the shaker and, therefore, separated from the reactor vessel. See more The well-defined and homogeneous liquid distribution during shaking is a key benefit of orbitally shaken single-use reactors. Reproducible flow … See more The applicability of orbitally shaken single-use reactors for aerobic bioprocesses highly depends on their potential to deliver a sufficient amount of oxygen to the cells. The first quantitative characterization of the oxygen transfer in … See more The first detailed characterization of the mixing performance in cylindrical orbitally shaken bioreactors with volumes ranging from 2 to 1,500 L was reported by Tissot et al. [29]. A calorimetric method was used to determine mixing … See more higgins lake state park michiganWebDec 20, 2024 · Orbitally shaken platforms can accommodate a wide range of vessel types and sizes, yielding a large degree of flexibility and independence from specific … higgins lake to crystal lakeWebMar 30, 2024 · Orbital shakers are simple devices that provide mixing, aeration, and shear stress at multiple scales and high throughput. For this reason, they are extensively used in a wide range of applications from protein production to … higgins lake state park southWebMar 1, 2024 · Disposable orbitally shaken bioreactors have been widely used for mammalian cell culture in suspension. Three kinds of baffle structures: vertical baffle, inclined baffle and horizontal baffle... higgins lake state park north vs south