Pentrtm/d-topotm 克隆试剂盒

Author: wltu

August undefined, 2024

Web6. nov 2014 · Store Box -20C.Item Concentration Amount pENTR vector,TOPO -adapted (pENTR 15-20ng/l linearized plasmid DNA 50%glycerol 50 mM Tris-HCl, pH 7.4 mMEDTA … Web1. jan 2014 · To generate the pDEST52-CYP 2 vector for the galactose-inducible expression of CYP 2, the full-length CYP 2 (CYP93E2, GenBank accession no. DQ335790) cDNA was amplified using primers 9 and 10 (see Table 1) and cloned into pENTRTM/D-Topo ® to produce pENTR-CYP 2. The cDNA was then transferred to pYES-DEST52 using Gateway …

前体、SARM的PCT，我有这些想法 - 知乎 - 知乎专栏

Web51TRF)观察GFP表达情况; 7 d后, 收集并提取重组农杆菌转化系(含AtRGS1-GFP融合基因)、对照(只含GFP基因)和空白对照(正常悬浮细胞系)的细胞总蛋白。10% SDS-PAGE电泳分离, 然后转膜(NC, Amersham公司)、漂洗、封闭, 一抗GFP 抗体(Santa cruz公司)孵育、洗膜、显色。 Webpentr™⁄sd⁄d-topo™ 克隆试剂盒含有 pentr⁄sd⁄d-topo™ 载体、dntp、盐溶液、无菌水、通用 m13 测序引物、oneshot™ top10 化学感受态大肠杆菌、s.o.c.培养基和 puc19 对照质 … fox nation education summit

pENTR-D-TOPO载体手册_文档下载

WebpENTR D-TOPO. Directional TOPO® cloning vector, for creating a Gateway® entry vector that is suitable for recombination with a destination vector. Sequence Author: Thermo Fisher … WebThe pENTR/D-TOPO Cloning Kits utilize a highly efficient, 5-minute cloning strategy (TOPO Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the … WebpENTR™/D-TOPO™ 克隆试剂盒，含 One Shot™ TOP10 化学感受态大肠杆菌. pENTR™/D-TOPO™ 克隆试剂盒，含 One Shot™ TOP10 化学感受态. 大肠杆菌. pENTR™/D-TOPO™ … fox nation download

Pentrtm 5 Topo Vector Thermo Fisher Bioz

Pentr 5 Topo Thermo Fisher Bioz

Web19. apr 2024 · (A, D) Comparison of axillary bud formation in rosette leaf axils of GA-related mutants della-p and ga1-3 to wild type plants. Ler and della-p were grown under SD for 4 weeks and then shifted to ... fox nation dogsWebInvitrogen 的pENTR™⁄D-TOPO® 克隆试剂盒采用高效的5分钟克隆策略 ("TOPO® Cloning") ，能够将平端PCR产物能以定向插入Gateway® 系统或多位点Gateway® 系统，平端PCR … fox nation election night

"WebEach Champion™ pET Directional TOPO™ Expression Kit is provided as a complete expression system. The Directional TOPO™ Expression box contains 200 ng of linearized, topoisomerase I-activated Champion™ pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and … " - Pentrtm/d-topotm 克隆试剂盒

Pentrtm/d-topotm 克隆试剂盒

pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 …

Web17. sep 2015 · Store Box -20C.Item Concentration Amount pENTR vector,TOPO -adapted (pENTR 15-20ng/l linearized plasmid DNA 50%glycerol 50 mM Tris-HCl, pH 7.4 mMEDTA mMDTT 0.1% Triton X-100 100 g/ml BSA 30 bromophenolblue 20 dNTPMix 12.5 mM dATP 12.5 mM dCTP 12.5 mM dGTP 12.5 mM dTTP water,pH SaltSolution 1.2 NaCl0.06 mlM13 … WebpENTR™/SD/D-TOPO™ 克隆试剂盒，包含 One Shot™ Mach1™-T1. R. 化学感受态. 大肠杆菌. 货号: K263520. 相关应用: Gateway Cloning. 技术支持客户服务. 货号. 单位规格.

Did you know?

WebVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. WebTOPOクローニングのテクノロジー Directional TOPOクローニング TOPOクローニング製品ガイド PCRクローニングにTaq DNAポリメラーゼまたはプルーフリーディング酵素のどちらを使用する場合でも、TOPO®クローニングキットを使用すれば、迅速かつ効率的にダウンストリームアプリケーションへと進むことができます。

Web丁香通-专业的科研采购信息和实验资讯平台 WebThe Directional TOPO™ Expression box contains 200 ng of linearized, topoisomerase I-activated Champion™ pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and an expression control. Store at -20°C.

http://www.haoranbio.com/ProductDetails-601088.aspx WebpENTR™⁄D-TOPO™ Cloning Kit contains pENTR⁄D-TOPO™ vector, dNTPs, salt solution, sterile water, universal M13 sequencing primers, OneShot™ TOP10 Chemically E. coli, … Simple Access to the Gateway™ System For access to the Gateway™ System, just … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes

Web传统/TA克隆. 5min Universal Ligation Mix. C311-01/02. T4 DNA Ligase. C301-01.

Web本试剂盒是利用拓扑异构酶(Topoisomerase)高效快速连接DNA片段的原理进一步研发而成，与传统的T4连接酶相比，具有以下优势：1）快速，仅需 1-5 min即可完成连接反应。 2）高效，无自连，阳性克隆率接近100%，无需设置蓝白斑筛选；3）操作简单，从连接到涂板仅需 15-20 min。操作过程省去冰浴、热激和1 h复苏。 4）可连接长达5 kb目的产 … fox nation elon muskWebpENTR/D-TOPO 克隆试剂盒利用高效的 5 分钟克隆策略（TOPO 克隆）将平末端 PCR 产物定向克隆成为进入 Gateway 系统或 MultiSite Gateway 系统的载体。平末端 PCR 产物以高 … fox nation elvisWeb24. sep 2013 · PENTRTM lentiviralvector( CS-RfA-EG) 最后，将 shRNA pCAG-HIVgp( 日本理化学研究所) ]导入到 293T 细胞中，制备表达携带 shRNA 慢病毒质粒的慢病毒液。这些慢病毒液即可直接用于感染周期性分裂期细胞。例如，用于感染原代细胞及未分化细胞时，需先 … fox nation end of menWeb5. jan 2014 · 唐雯，等.人组织因子基因RNAi 载体pENTR TM /U6-TF-shRNA 的构建与鉴定 ISSN 1673-8225 CN 21-1539/R CODEN: ZLKHAH 259 www.zglckf.com 中山大学附属第一医院儿科, 广东省广南方医科大学南方医院检验医学中心，广东省广州方医科大学中医药学院中医症候实验室，广东省广庆市人 ... black walled houseWebThermo Fisher pentrtm sd d topotm vector Pentrtm Sd D Topotm Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed … black walled bedroomWeb25. dec 2024 · 二十年前，TOPO 之名就已成为 PCR 克隆领域高质量和颠覆性创新的代名词。如今，Invitrogen TOPO 克隆试剂盒仍然是最快捷、最简单、最高效的克隆解决方案。无可取代。TOPO 克隆技术手册快捷—仅需 5 分钟，室温下反应简易—只需简单 3 步，即可向您的 PCR 产物之中加入限制性酶切位点或通用引物位点 ... fox nation eventWebpENTR™/TEV/D-TOPO™ 克隆试剂盒，含 One Shot™ TOP10 化学感受态. 大肠杆菌. 货号: K252520. 相关应用: Gateway Cloning. 技术支持客户服务. 货号. 单位规格. 价格（CNY）. … black wall elbow